The aim of this study was to establish knowledge about the most suitable substrate for cultivating different kinds of anaerobic beer spoilage bacteria in the brewing industry. During the study, two other aspects were investigated; if the sample volume affects the results and which anaerobic beer spoilage bacteria are present in the micro flora at the brewery. The method used was standard cultivation with four different substrates; Universal Beer Agar, Nachweismedium Bierschädliche Bakterien, Raka Ray and VLB S7-S. During the identification of bacteria in the micro flora, a method using PCR was used. None of the substrates showed a significant advantage over the others.

The aim of this study was to isolate and identify Lactobacillus species indigenous to the gastrointestinal microflora of Swedish horses. These lactobacilli are to be used as probiotic candidates in research concerning antibiotic-associated diarrhoea in horses. Eight horses, age 6-10 years, were selected from a riding school for collection of faeces samples. Man Rogosa Sharp medium and gram staining were used for the selection of supposed lactobacilli. We could identify 33 isolates of supposed lactobacilli. With the use of API tests 22 of these 33 isolates could be identified.

The purpose of the project was to exam steams ability to sterilize carrots infected withLactobacillus plantarum. The project was a cowork with Brämhults Juice AB. They hadobserved an increase of Lactobacillus in the production of carrot juice during the summer. Tokeep the flavour of fresh fruits, the juice is treated carefully with heat. This means that thejuice only stays fresh for a few days, around two weeks for an unopened package and threedays for an opened package.In an attempt to remove the remaining Lactobacillus of the carrots, steam is tested as an extrastep in the production of juice.

Lactobacillus reuteri has been used as probiotics in piglet and chicken feedings as well as in different diary products for humans. In this EEF-work I have shown that L. reuteri can be an alternative for treatment of vaginitis in laboratory rats used for breeding. In a breeding stock with 3000 animals some animals had a vaginal infection with ??haemolytic streptococci group G.

Detta examensarbete redogör för en enzymatisk metod för att analysera den protolytiska aktiviteten i Enzobact. Enzobact är en ostmognadskultur,som framställs genom värmebehandling av Lactobacillus helveticus, och tillämpas vid ystning av lågfetthaltiga ostar. Det är lämpligt att en ostmognadskultur har en låg protolytisk aktivitet för att den färdiga produkten ska ha samma smak och utseende som en normalfet ost..

The probiotic bacteria Lactobacillus reuteri DSM17938 was evaluated as a starter culture in cheese production, with the intention to produce a new interesting product within the functional food market. Growth and acidification rate in milk subjected to different heat treatments, as well as supplementation with growth promoting factors or support cultures was measured during controlled fermentations. The results showed that the weak proteolytic activity of L. reuteri could be compensated for by supplementation with 1% casamino acids or 5% MRS broth. Furthermore, a decrease in growth and acidification rate could be seen when using yoghurt culture or Lactobacillus delbrueckii as adjunct cultures.

När man bakar ett surdegs bröd är det viktigt att hålla degens egenskaper konstanta så att de inte ändras från dag till dag. Detta går att underlätta genom att använda sig av en starterkultur. Beroende på vilken kultur man använder sig av får degen dess unika egenskaper. Man kan till exempel använda sig av en bakteriocinproducerande bakteriekultur som stabiliserar degen.I detta examensarbete försöktes tillväxten av Lactobacillus gasseri 42 att optimeras. L.

About 10 % of the adult women population in Sweden are treated annually for urinary tract infections. The increasing bacterial resistance towards antibiotics is classified by WHO (World Health Organization) and ECDC (European Centre for Disease Prevention and Control) as one of the greatest treats for human health in a global perspective. To find alternatives scientists are studying the possibility to use probiotics to reduce the frequency of recurring urinary tract infections. This literature study examines five randomized double blinded placebo controlled studies where different strains of Lactobacillus have been used as a prophylactic to women suffering from recurrent urinary tract infections and bacterial vaginosis. Overall the studies do not display enough promising results to recommend the use of probiotics as a prophylax or cure..

Mjölksyrabakterier tillhörande släkten Lactobacillus och Bifidobacterium har nyligen upptäckts hos bin och i honungen de producerar och innefattar 13 nya arter[1]. Forskarna arbetar med att ta fram nya snabba och mer pålitliga identifierings metoder för att karakterisera dessa bakterier. I detta projekt undersöktes möjligheten att identifiera dessa bakterier med en ny metod som heter denaturing high performance liquid chromatography (DHPLC). Metoden bygger på separation av PCR (polymerase chain reaction) amplifierade 16S rDNA fragment i DHPLC [8]. Vid identifiering av bakterier amplifierades olika variabla regioner från 16S rRNA genen, som påvisade efter sekvensering störst genetisk variation mellan dessa bakterier [1]. Separationen utfördes med ion-pair reverse-phase high presure liquid chromatoghaphy (IP RP HPLC) med delvis denaturering av DNA molekylen.

Abstract Cavities used to be a very common problem but over time humans have learned to take care of their teeth. However, there are still many who have problems. This problem is due to microorganisms and in particular the various streptococci and lactobacilli producing acids which loosens up the enamel on teeth. These bacteria can be found at various places in the mouth and saliva. Besides being a discomfort it can be a major problems and the damaged tooth needs to be removed or make a dental filling. The aim of the study was to see how intake of probiotic tablets containing Lactobacillus reuteri PTA ATCC 5289 and Lactobacillus reuteri DSM 17938 affects the levels of oral Streptococcus mutans and lactobacilli over a three-week period.

Examensarbetet utfördes på SP-Food and Bioscience (tidigare SIK-Institutet för Livsmedel och Bioteknik) och anknyter till projektet DynahMat (Dynamisk Hållbarhetsdatum för Minimerat Svinn). Syftet med examensarbetet var att undersöka hur väl tillväxten av mjölksyrabakterier predikteras av tre befintliga prognosmodeller jämfört med tillväxten av naturligt förekommande mjölksyrabakterier i MA-packad rökt skinka.Tillväxten av mjölksyrabakterier i rökt MA-packad skinka studerades vid tre lagringstemperaturer (4, 8 respektive 12 °C) och resultat visade att förskämningen av den rökta skinkan skedde snabbare vid en högre lagringstemperatur. De befintliga prognosmodellerna som tillämpades var modeller framtagna av Kreyenschmidt et al. (2010), Mataragas et al. (2006) och Devlieghere et al.

Denna examensrapport redogör för en metodutveckling av aminotransferase aktivitet som är utförd för Medipharmv AB i Kågeröd som tillverkar Enzobact. Enzobact är en bakteriekultur som består av värmebehandlad Lactobacillus helveticus och används vid tillverkning av lågfetthaltiga ostar, vilket bidrar till att osten får en bättre smak och konsistens..

Halten näringsämnen i livsmedel varierar bland annat med tillagningsmetod, lagringstid och lagringsförhållanden, som exponering för ljus och syre. Oklarheter finns för mikrovågors inverkan på näringsämnen och framförallt på vitamin B12. Eftersom många äldre drabbas av brist på vitamin B12, är detta av intresse eftersom många hemmaboende äldre människor får hemleverans av färdiga matportioner som är avsedda att värmas, ofta i mikrovågsugn. Syftet med studien var att kunna ge en indikation på om uppvärmning genom mikrovågor påverkar halten vitamin B12 i färdiga kylda måltider. Detta gjordes genom litteratursammanställning om vitamin B12 och mikrovågar samt genom bioassay med Lactobacillus delbrueckii.

The purpose of this study was to investigate whether resistant maltodextrin is suitable as a fiber source in the probiotic fruit drink ProViva Mango without affecting the probiotic bacterium Lactobacillus plantarum 299v, viscosity or the sensory experience of the drink. Resistant maltodextrin is produced from hydrolyzed corn starch and is classified as a soluble dietary fiber. It is relatively resistant to digestion in the small intestine, where approximately 10% can be broken down, 50% can be fermented in the colon by the intestinal flora and 40 % is excreted in the faeces. Resistant maltodextrin has several important qualities when it comes to food processing. When added in a product, it does not affect the viscosity or taste and it is heat and acid stable.

The bacterium Lactobacillus reuteri, which natural habitat is in the intestine of mammals and birds, uses glycerol as a substrate for production of the antimicrobial compound reuterin. Glycerol has been shown to decrease the number of Escherichia coli in human feces and it is believed to be due to in situ production of reuterin. E. coli belongs to the large family of Enterobacteriaceae, naturally occurring in the intestine, and pathogenic strains of E. coli have been shown to be one of several bacteria causing diarrhoea in calves.